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Biol Reprod 80(5):913919 Zhang M, Jiang M, Bi Y, Zhu H, Zhou Z, Sha J (2012) Autophagy and apoptosis act as partners to induce germ cell death after heat stress in mice

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After dehydration with ethanol and propylene oxide and embedded in polybed 812 resin (Polysciences, 025950-1), ultrathin (80 nm) sections were sectioned with 2% uranyl acetate and stained with 0.3% lead citrate and subjected to preparation for TEM (JEM-1400plus, Japan) observation

All these observations were confirmed and supported by in vitro experimental data, including evaluation of cytotoxic effects on healthy RBCs [269,270,271]

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