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It plays a role in metabolism and cellular signalling

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[DOI] [PubMed] [Google Scholar] 77.Giacco A, Delli Paoli G, Senese R, Cioffi F, Silvestri E, Moreno M, et al

For transduction, an overnight culture of the recipient strain (200 l) was mixed with CaCl 2 (28 l 50 mM) and P1 lysate (50 l) of the donor strain and incubated for 20 min at 37C