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Amsterdam, The Netherlands: 2014
One study has shown that kisspeptin administration can substantially decrease central levels of the key inhibitory neurotransmitter GABA in the human brain, leading to changes in how the body reacts to anxiety, stress, and fear [1]
The cDNA was then fourfold diluted and used as a template in quantitative PCR with IQ SYBR Green Supermix (Bio-Rad) on a CFX96 instrument (Bio-Rad) using a 3-step protocol
PubMed PMID: 8120456
Also in this work, whole blood was mixed with a precipitating solution containing both NEM and sulfosalicylic acid
