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Possible causes: dead space accumulation (discussed above), inaccurate water volume at reconstitution (more water means more dilute, means more volume per dose, means fewer doses from the same amount of peptide), or air in the vial displacing solution

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The vectors further contained CMV enhancer and promoter supporting expression in HEK293E cells

The counteracted effects of lidocaine and bupivacaine [60,89] can suggest an extended effect even beyond the prime local anesthetic effect