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HED/Cys-SG and CHP assays If not otherwise indicated, strep-MBP-GRX samples were reduced with 10 mM DTT for 2 hours and the buffer was exchanged against 100 mM Tris/HCl p H 8.0, 1 mM EDTA 15 using PD MiniTrap G-25 desalting columns (GE Healthcare)

et al., 2021) that emodin mediates autophagy through the AMPK/mTOR signaling pathway, thereby reducing apoptosis and podocyte injury in DKD rats

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These transcription factors increase the expression of Tyrosinase (TYR), a key enzyme involved in melanin synthesis, thereby stimulating melanin production (94, 95)

R.AlibolandiZ.SarkarP.GhazanfariM.Azami TamehA.et al (2021)