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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)

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This was done by encapsulating curcumin in a self-assembled manner into a metal-polyphenol network formed by the complexation of EGCG with Fe 3+ , and then using yeast microcapsules for piggybacking, which cleverly repaired the intestinal microenvironment through the triple pathway of antioxidant, inhibition of inflammation, and regulation of intestinal flora [53]

DTNB (5-5-dithiobis [2-nitrobenzoic acid) reacts with GSH to form yellow color chromphore, 5-thionitrobenzoic acid (TNB) with absorbance maxima at 415 nm and GS-TNB