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Cagrilintide supplied for research purposes is manufactured as a high-purity, LPS-free peptide and is endotoxin-free , ensuring suitability for sensitive laboratory applications

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For the Sholl analysis, a mixed-effect repeated measure ANOVA was used to analyze the number of intersections per radius step

Western blot analysis of thoracic aorta proteins showed that the classic aging markers P21 and P16 were significantly downregulated following FOXO4-DRI treatment, whereas the expression levels of the cell proliferation marker Ki-67 and Lamin B, a key protein involved in nuclear structural stability, were markedly upregulated (Figure 1A)