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Cellular debris was removed by centrifugation at 10,000 g for 20 min and the membranes harvested by centrifugation at 100,000 g for 1 h

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77 In contrast, endothelial dysfunctionan early and independent predictor of cardiovascular eventscontributes to both macrovascular and microvascular pathology, playing a central role in the progression of diabetic CVD 78,79 (Fig
