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doi: 10.1038/nature17393 103 MetalloCMGameiroPABellELMattainiKRYangJHillerKet al

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Lukomska B, Stanaszek L, Zuba-Surma E, Legosz P, Sarzynska S, Drela K (2019) Challenges and controversies in human mesenchymal stem cell therapy

Correct insertion yields a novel NcoI site generated by ligation of the blunted ends, and so NcoI (NEB) linearization of the supercoiled vector was used to determine correct insertion (Figure 1b, lower panel)