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K., & Kang, N

Simultaneously, the reaction was held without GSSG addition

By maintaining excess GR, hydroperoxide, GSH, and NADPH, the reaction rate becomes solely dependent on GPx activity, enabling continuous, real-time kinetic measurement (Figure 2C)

10.2174/1570159X14666160229114755 3 BarnettR

This means the reconstitution volume choice also affects how quickly the vial needs to be used
