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To assess the effect of amino acids on TC1-6 cells, cells were cultured in MEM (Gibco, 11090-081) supplemented with 5% FBS, 1% Penicillin/Streptomycin, 4 mM glutamine and 4 mM alanine

Always factor this into your experimental design

For a real-world illustration of how these variables interact, our piece on how long 10mg of retatrutide lasts walks through similar concentration-based thinking applied to a different compound

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Molecular mimicry between bacterial antigens and self-antigens may initiate or perpetuate autoimmune responses