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For mitochondrial functionality, the model included group (CON or CAR), time (relative to LPS injection), stimulus (unstimulated, ConA or LPS) and their interactions as fixed factors

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Cell apoptosis analysis Apoptosis was assessed by flow cytometry using a KeyGEN Annexin V-FITC Apoptosis Detection Kit (KeyGEN BioTECH, China) according to the manufacturers instructions
