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Youll wash the column to get rid of as many contaminating proteins as possible, then elute the tagged proteins by adding a buffer component that interferes with the ligand-tag interaction (Figure 6)

IL-17, which has a variable inflammatory effect that promotes the migration of inflammatory cells to inflammatory tissues, levels of IL-17 were found to decrease in lung homogenates of fibrotic mice treated with vitamin C (126)

BMJ Open Respir Res

A 100 L volume of supernatant was transferred to a microtube and mixed with 100 L of 2, 4 nitrophenylhydrazine (DNPH) prepared as a 5 mM solution in 2 M hydrochloric acid