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After alignment, reads were filtered to include only cells with: (i) a minimum of 100 genes detected, (ii) each gene detected in a minimum of 3 cells, and (iii) cells containing 1% mitochondrial DNA
Follistatin-like 1 (FSTL1) is a prognostic biomarker and correlated with immune cell infiltration in gastric cancer

Subsequently, the Amplex Red reaction mixture containing 100 M Amplex Red reagent and 0.2 U/mL horseradish peroxidase in KRPG buffer was added at 50 L to each well, and the fluorescent intensity was determined using SpectraMax ID5 microplate reader with excitation at 535 nm and emission at 590 nm at the indicated time points
Plant Physiol Biochem 157:138147 Festa RA, Thiele DJ (2011) Copper: an essential metal in biology
Can GHK-Cu Cream be used with other skincare products