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Unnatl Amino Acids Methods Protoc 794:135167

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On the second day, the sections were incubated with a secondary antibody (Alexa Fluor 488 rabbit anti-mouse IgG (1:500, Invitrogen, Carlsbad, CA, USA)) for 60 min, followed by a counterstain with propidium iodide and mounting using glycerol

183 In addition, Small molecule antagonists which inhibit TGF- receptor kinase or TGF- activated pathways were studied, 184,185 although the long-term safety of such drugs might be a problem, particularly as TGF- affects tissue repair and is a potent anti-inflammatory mediator (Table 3)