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While most published animal studies use once-daily dosing, some research protocols investigate twice-daily administration, particularly for acute injury models or when mimicking the continuous exposure that might occur with oral dosing

Statements Author contributions AR and JA contributed to the organization and design of the manuscript, performed literature reviews, participated in drafting sections of the manuscript, and in revising, reading, and approving the final submitted version

Several alternatives target tissue repair through different mechanisms

Moderate, regular exercise supports treatment outcomes better than extreme regimens

Microneedling combined with topical GHK-Cu is another approach, creating tiny channels through the stratum corneum that allow the peptide to reach the dermis