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For F-actin quantification, at the end of experiment, cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phallodin 1 g/mL (Sigma Aldrich) during 5 min

The lengths (L) and widths (W) of the tumors were measured, and the tumor volumes were calculated as (L W 2 )/2

PMID 28938471

ELISAs can be incorporated to focus on immune specific interactions to assess the active concentration of target molecules

Average milk levels in mature milk are 200 to 700 pmol/L (270 to 950 ng/L) during the first 28 weeks postpartum in healthy, lactating women with known or presumed adequate vitamin B 12 status and not taking a supplement.[27,28,42-45] Levels are highest during the first 4 weeks postpartum and gradually decrease thereafter.[25,28,29,42-47] Hindmilk levels are 20% to 30% higher than foremilk.[29] In populations with a high prevalence of vitamin B 12 deficiency or insufficient dietary vitamin B 12 intake, reported median or average milk levels are 120 to 160 pmol/L (160 to 220 ng/L) at 6 months postpartum.[30,46] In one study of 210 lactating women from different regions of Cameroon, rates of maternal vitamin B 12 deficiency were 15% to 25% and median milk levels were between 47 and 236 pmol/L (64 and 320 ng/L)