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2.8 Quantitative evaluation of superoxide anion production in 1-DNJ treated cell lines using the NBT assay To quantitatively assess the intracellular superoxide anion production, the Nitro Blue Tetrazolium (NBT) reduction assay was utilized following the methodology of 2.9 ABTS + antioxidant ability The scavenging activity of 1-DNJ against ABTS + cation radicals was assessed, and the data were obtained through spectrophotometry using the SpectraMax i3 ELISA reader

doi:10.2478/rjim-2023-0001

Gastrointestinal: Nausea, cholestatic jaundice, alterations in liver function tests, rarely hepatocellular neoplasms and peliosis hepatis (see WARNINGS)