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The monocytes (2x10 6 )/well were seeded in wells of six-well plates and cultured in a nutrient-rich culture medium (RPMI1640 medium supplemented with 10% calf serum, 2 mM mercaptoethanol, 1 mM glutamine, 100 unit/ml penicillin G, and 100 g/ml streptomycin sulphate) and a DC growth stimulant mix of GM-CSF and IL-4

Abbreviations PH: partial hepatectomy GST: glutathione S- transferase GSH: glutathione TRAF2: tumor necrosis factor receptor-associated factor 2 ASK1: apoptosis signal regulating kinase 1 MAPK: mitogen-activated protein kinase JNK: c-jun N-terminal kinase ERK: extracellular signal regulated kinase MCM: minichromosome maintenance ROS: reactive oxygen species BrdU: 5-bromo-2'-deoxyuridine ATF: activating transcription factor CDK: cyclin-dependent kinase ALT: alanine aminotransferase AST: aspartate aminotransferase References Fausto N, Campbell JS, Riehle KJ

Xu T et al (2023) Single-cell profiling reveals pathogenic role and differentiation trajectory of granzyme K+CD8+ T cells in primary Sjgrens syndrome

PMID: 88 JiangKLiJJiangLLiHLeiL

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