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ASD might have undergone a complex sequential hydrolysis process in vivo , which might have contributed partly to systemic clearance

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were significantly inhibited by -sitosterol in the in vitro investigation, with an IC 50 of 55 and 50 g/mL, respectively
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To eliminate potential genomic DNA contamination, the extracted RNA was treated with DNase I (RNase-free, Thermo Fisher Scientific, USA)
