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10.1016/j.febslet.2012.08.017 28 MukherjeeK.ParashuramanS.KrishnamurthyG.MajumdarJ.YadavA.KumarR.et al (2002)

The dried samples were reconstituted using 200 HPLC mobile phase (10% v/v ACN in water), vortexed briefly, and subjected to HPLC separation and analysis

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Apply one to two pumps to clean skin
