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Serum cobalamin levels do not strictly correlate with physiologic deficiency, in part because traditional assays measure total serum cobalamin, even though the fraction bound to transcobalamin II (holotranscobalamin II) is physiologically most important

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The frequency of the vitamin B12 injections depends solely on the individuals health needs, such as age, underlying cause of malabsorption, dietary habits, medication and most importantly, response to the injection