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Under normal conditions, NRF2 is bound in the cytoplasm to its inhibitor KEAP1, which targets it for degradation

Interestingly, IGF-1 has been found to play a compensatory role for both estrogen receptor (ER) and androgen receptor (AR) by augmenting hormonal responses in the absence of, or where low levels of ligand are present

As was previously mentioned, levels of GH and IGF-1 steadily decrease over a lifespan, and with the lower hormone levels comes a myriad of side effects such as increased fat mass, cardiovascular dysfunction, decreased muscle mass, and declined cellular protein synthesis

We recommend waiting a minimum of 60 minutes, but for optimal results, a 90 to 120-minute window is best

We also analyzed changes in nuclear factor B (NF-B) and cyclooxygenase-2 (COX-2) expression levels to delineate possible mechanisms of their combined action
