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Recent B12-Rich Meal (SMALL EFFECT) High B12 foods and content: Beef liver (100g): 60-100 mcg (extreme!) Clams (100g): 80-100 mcg Beef steak (200g): 5-6 mcg Salmon (100g): 3-4 mcg Milk (1 cup): 1 mcg Eggs (1 large): 0.6 mcg Liver Special Case: Eating liver the day before the test can significantly increase B12 Avoid liver 3-7 days before testing Regular meat: Single steak: small effect (~10-20 pg/mL increase at most) Probably not clinically significant REAL-WORLD CLINICAL IMPACT Case Example 1: Borderline Result Patient A: Baseline B12: 210 pg/mL (borderline deficient) Ate a large steak 18 hours before the test Possible effect: The result might have been 190-200 pg/mL without the meal Clinical impact: Could change interpretation from borderline to clearly deficient. Conclusion: In borderline cases, recent meat consumption could mask deficiency Case Example 2: Clear Deficiency Patient B: Baseline B12: 120 pg/mL (clearly deficient) Ate steak 18 hours before the test Possible effect: Might increase to 135-145 pg/mL Clinical impact: NONE still clearly deficient Conclusion: When B12 is truly low, a single meal wont mask it Case Example 3: Clear Adequacy Patient C: Baseline B12: 550 pg/mL (clearly adequate) Ate steak 18 hours before the test Possible effect: Might increase to 580-600 pg/mL Clinical impact: NONE still clearly adequate Conclusion: When B12 is adequate, a meal doesnt change interpretation EXPERIMENTAL STUDIES Study 1: Acute B12 Loading Design: Gave healthy volunteers 10 mcg oral cyanocobalamin Measured serum B12 at baseline, 2h, 4h, 8h, 24h Results: 2 hours: No significant increase 4 hours: Minimal increase (~5-10%) 8 hours: Peak increase (~15-20%) 24 hours: Still elevated (~10-15% above baseline) Conclusion: Even a pharmacologic dose (10 mcg, much more than food) produces a modest, delayed increase Study 2: Meal Effect Study Design: Measured B12 before and after a standardized meal Meal contained ~3 mcg B12 (typical) Results: 1 hour post-meal: No change 4 hours post-meal: Small increase (mean 8%) 8 hours post-meal: Peak increase (mean 12%) Conclusion: A single meal produces a small, delayed effect CLINICAL RECOMMENDATIONS For Most Accurate B12 Testing: DO: Fast overnight (8-12 hours) if possible though not mandatory Morning testing (standardization) Stop B12 supplements 7 days before the test Stop multivitamins 2-3 days before the test Avoid liver and organ meats 3-7 days before the test Avoid energy drinks/fortified foods the day before the test Note any recent B12 injections (within 2 weeks) DONT: Take a B12 supplement on the day of the test Eat liver the day before the test Drink energy drinks before a test Take multivitamins the morning of the test LESS CRITICAL (Small Effect): Eating regular meat (steak, chicken, fish) the day before Drinking milk Eating eggs ANSWERING YOUR SPECIFIC QUESTION If I test, eat meat, and immediately retest? Answer: Within 30 minutes to 2 hours: Virtually NO DIFFERENCE Within 4-8 hours: Small increase possible (5-15%) Within 12-24 hours: Modest increase possible (10-20%) But the effect depends on: Amount of B12 in meat Your baseline B12 status Your absorption capacity Individual metabolism BOTTOM LINE Summary Table: Effect of Meat on B12 Testing Practical Takeaway: For your specific question: If you test B12, immediately eat meat, and retest 30 minutes later, Results will be virtually identical

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Increased Muscle Hypertrophy Lastly, but most importantly, peptides play a crucial role in promoting muscle hypertrophy - the process of growth and increase of the size of muscle cells

Show Notes Emerging Use of BPC-157 in Orthopaedic Sports Medicine: A Systematic Review Transcript Mike Reinold: Welcome back, everybody, to the latest episode of the Ask Mike Reinold Show

The 5 initial loading doses are usually all that is needed for long lasting protection