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For immunofluorescence, cryosections were dried for 2 h at RT, fixed in pure acetone for 10 min and blocked with PBS 10% FCS for 30 min at RT

Updated insights into the molecular networks for NLRP3 inflammasome activation

The therapeutic index is compromised because normal tissues, such as bone marrow, the gastrointestinal mucosa, and hair follicles, receive exposure equivalent to the tumor

Abbreviations Used -KG -ketoglutarate 6PGD 6-phosphogluconate dehydrogenase AGC1 aspartate-glutamate carrier 1 Ang II angiotensin II AOA aminooxyacetic acid DHAP dihydroxyacetone phosphate eIF2 eukaryotic initiation factor 2 ER endoplasmic reticulum F6P fructose-6-phosphate FH fumarate hydratase FTHFDH 10-formyl-tetrahydrofolate dehydrogenase G-3-P glyceraldehyde-3-phosphate G-6-P glucose-6-phosphate G6PD glucose-6-phosphate dehydrogenase GAPDH glyceraldehyde-3-phosphate dehydrogenase GCL -glutamylcysteine ligase GGT -glutamyl transpeptidase GLS glutaminase GLUD glutamate dehydrogenase GOT glutamate-oxaloacetate transaminase GPDH glycerol-3-phosphate dehydrogenase GPx glutathione peroxidase GR glutathione reductase Grx glutaredoxin GS GSH synthetase GSH glutathione GSSG GSH disulfide H 2 O 2 hydrogen peroxide HIF-1 hypoxia-inducible factor 1 Hsp27 heat shock protein 27 IDH isocitrate dehydrogenase IR ischemia/reperfusion KGDH -ketoglutarate dehydrogenase LDH lactate dehydrogenase L2HG l-2-hydroxyglutarate MDH2 malate dehydrogenase 2 ME malic enzyme MTHFD methylene-tetrahydrofolate dehydrogenase NAC N -acetyl-l-cysteine NAD + nicotinamide adenine dinucleotide NADH reduced NAD + NADK NAD + kinase NADP + phosphorylated NAD + NADPH reduced NADP + NAM nicotinamide NNT nicotinamide nucleotide transhydrogenase NR nicotinamide riboside NOX NAD(P)H oxidase NRF2 nuclear factor (erythroid-derived 2)-like 2 O 2 superoxide anion OAA oxaloacetate OXPHOS oxidative phosphorylation PDH pyruvate dehydrogenase PHD2 prolyl hydroxylase domain-containing protein 2 PPP pentose phosphate pathway Prx peroxiredoxin PSSG protein-glutathione disulfide ReBC redox buffer capacity RNS reactive nitrogen species ROS reactive oxygen species SDH succinate dehydrogenase SIRT sirtuin deacetylase SOD superoxide dismutase TCA tricarboxylic acid THF tetrahydrofolate TR thioredoxin reductase Trx thioredoxin xCT cystine transporter References 1

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