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The samples were shaken for 30 min at 40 C

Glutathione is a powerful antioxidant naturally produced in every cell of the human body

Receiver Operating Characteristic (ROC) analysis revealed that vanillic acid and N-hydroxy-isoleucine were the highest discriminative metabolites with an AUC value of 1 and also 2-aminobenzoic acid and picolinic acid showed high discriminative ability with an AUC of 0.995 and these might serve as potential prognostic biomarkers to monitor the progression of CKD to ESKD (Dahabiyeh et al., 2023)

Lu C, Han HD, Mangala LS, Ali-Fehmi R, Newton CS, Ozbun L et al

The Role of Glutathione in Mitochondrial Defense The primary reason to use glutathione for athletes is that it neutralizes ROS before they can destabilize the energy-producing chain