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The wounds pictures were taken over the certain days and the remaining wound areas percentage was measured according to the following equation: Histopathology study Animals on mentioned days were sacrificed by ketamine overdose injection, and the collected tissues were placed in a 10% formalin solution and fixed for 24 h

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MTHFR Gene Polymorphism Is Associated With DNA Hypomethylation and Genetic Damage Among Benzene-Exposed Workers in Southeast China Journal of Occupational and Environmental Medicine 60.4 (2014): e188-e192 Nikesj, F, et al

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The quantitative detection of the expression of proteins related to the conduction of the PI3K/Akt pathway, such as p-Akt, p-PI3K, Bcl-2, and Caspase-3, can provide objective and quantifiable biological indicators for the effect of TCM intervention