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After identifying the optimal separation and detection conditions, the calibration curves for GSH and GSSG were measured within the concentration range of 0.2100 M

H 2 O 2 quantification within the Joint Cavity of Recurrent Gouty Rats A Hydrogen Peroxide assay kit (A064-1-1, Nanjing Jiancheng Bioengineering Institute) was utilized to quantify the H 2 O 2 concentration in the joint cavity of the rats, wherein H 2 O 2 interacted with molybdic acid to generate a complex exhibiting maximum absorbance at 405 nm

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an equivalent H 2 O (10 stock) buffer was made at pH 7.4