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The column was washed with 50 mM Tris-Cl, pH 8.0 and 250 mM NaCl

However, with the rise of intolerance (IBD/IBS), allergens, dietary preferences, and or restrictions have increased the demand for alternative solutions to the once uncontested animal-based proteins

doi:10.1016/j.molmet.2013.05.006 68

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Metabolic engineering of the omega-3 long chain polyunsaturated fatty acid biosynthetic pathway into transgenic plants