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10.3390/ijms20112723 76 ManciasJ
Msaouel, P
Nusinersen improves walking distance and reduces fatigue in later-onset spinal muscular atrophy
Co-Immunoprecipitation and Immunoblotting Analysis Cells were washed twice with phosphate-buffered saline (PBS), and then lysed in a lysis buffer on ice for 30 min, and then cell lysates were centrifuged at 12,500 rpm for 15 min
