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Post-induction the cells were pelleted down at 6,000 x g for 15 min at 4C and the pellets were resuspended in 4XT (200 mM Tris, pH 8.0, 800 mM NaCl, 1 mM DTT) supplemented with protease inhibitors (1 mM AEBSF, 0.8 M Aprotinin, 15 M E-64, 10 g/mL Bestatin, 10 g/mL Pepstatin A and 10 g/mL Leupeptin) and lysed by sonication

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Collagen turnover in normal and degenerate human intervertebral discs as determined by the racemization of aspartic acid

Pinus pinaster is a type of pine tree native to areas of the Mediterranean including Portugal, Spain, France, and Morocco

Zhou C, Huang Y, Wang D, et al