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Paclobutrazol exposure induces apoptosis and impairs autophagy in hepatocytes via the AMPK/mTOR signaling pathway

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Frozen hepatic samples were lysed using Qiazol reagent, and total RNA extraction was done with a QIAGEN RNAeasy Mini Kit (Hilden, Germany)

Extracellular cysteine can also directly be taken up by cultured astrocytes and used for GSH synthesis, but its disulfide cystine, that is transported into rat astrocytes mainly by the cystineglutamate antiporter X C , serves as the preferred extracellular substrate for the cytosolic cysteine needed for astrocytic GSH synthesis (Kranich et al