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It's the single most important rule of this entire protocol
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After transfer, membranes were blocked in 1% bovine serum albumin in PBS with 0.1% Tween-20 and probed with anti-PARP (NB100-56599, Novus Biologicals, Littleton, CO), anti-Glutathione (AB19534, Abcam, Cambridge, MA), anti-phospho-IB- (9246), anti-IB- (9242), anti-phospho-IKK- (2594), anti-IKK- (2370), or anti-p65 (8242) (Cell Signaling Technology, Danvers, MA) antibodies

2009 Aug 5;1790(10):114960

Chu, C., Tsuprykov, O., Chen, X., Elitok, S., Krmer, B
